منابع مشابه
Rabbit Liver Guanine Deaminase
Homogeneous guanine deaminase (guanine aminohydrolase, EC 3.5.4.3) is a single polypeptide chain of mean molecular weight of 55,000, as determined by Sephadex gel filtration and sodium dodecyl sulfate polyacrylamide disc gel electrophoresis. Irreversible inactivation was observed upon incubation with P-chloromercuribenzoate, at a concentration of 1.0 x lop4 M. Iodoacetic acid and iodoacetamide ...
متن کاملIdentification, expression, and characterization of Escherichia coli guanine deaminase.
Using the human cDNA sequence corresponding to guanine deaminase, the Escherichia coli genome was scanned using the Basic Local Alignment Search Tool (BLAST), and a corresponding 439-residue open reading frame of unknown function was identified as having 36% identity to the human protein. The putative gene was amplified, subcloned into the pMAL-c2 vector, expressed, purified, and characterized ...
متن کاملGuanine-deaminase Activity in Rat Brain and Liver.
1. Guanine deaminase in rat brain and liver was distributed among all the subcellular fractions: nuclei, ;heavy' mitochondria, ;light' mitochondria, microsomes and the supernatant fluid. The greater part of the activity passed into the soluble fraction. Among the particulate components, the ;light' mitochondria constituted the richest fraction. 2. The sum of the enzymic activities of the compon...
متن کاملOxidative modulation and inactivation of rabbit cardiac adenylate deaminase.
Oxidative stress and adenine nucleotide catabolism occur concomitantly in several disease states, such as cardiac ischaemia-reperfusion, and may act as synergistic determinants of tissue injury. However, the mechanisms underlying this potential interaction remain ill-defined. We examined the influence of oxidative stress on the molecular, kinetic and regulatory properties of a ubiquitous AMP-ca...
متن کاملInduction of guanine deaminase and its inhibitor in rodent liver and brain.
1. Guanine deaminase activities in homogenates and supernatant fractions of liver and brain of rat and mouse were elevated by administration of guanine to the animals. The maximum induction in mouse tissues occurred within 24h and in rat tissues within 48h. 2. Mitochondria of rat (but not mouse) liver and brain contain an inhibitor of supernatant guanine deaminase, and this was also increased b...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1973
ISSN: 0264-6021
DOI: 10.1042/bj1310683